PoplarV1, Main, Exploration, bibRecord, 002703

Enhancement of production of eugenol and its glycosides in transgenic aspen plants via genetic engineering.

Identifieur interne : 002703 ( Main/Exploration ); précédent : 002702; suivant : 002704

Enhancement of production of eugenol and its glycosides in transgenic aspen plants via genetic engineering.

Auteurs : Takao Koeduka [Japon] ; Shiro Suzuki ; Yoko Iijima ; Toshiyuki Ohnishi ; Hideyuki Suzuki ; Bunta Watanabe ; Daisuke Shibata ; Toshiaki Umezawa ; Eran Pichersky ; Jun Hiratake

Source :

Biochemical and biophysical research communications [ 1090-2104 ] ; 2013.

RBID : pubmed:23707945

Descripteurs français

English descriptors

KwdEn :
- Acetyltransferases (genetics), Acetyltransferases (metabolism), Alkenes (chemistry), Chromatography, Gas (MeSH), Eugenol (metabolism), Gene Expression Regulation, Plant (MeSH), Genetic Engineering (MeSH), Glycosides (metabolism), Oxidoreductases Acting on CH-CH Group Donors (genetics), Oxidoreductases Acting on CH-CH Group Donors (metabolism), Plant Leaves (metabolism), Plant Proteins (genetics), Plants, Genetically Modified (metabolism), Populus (metabolism), Tandem Mass Spectrometry (MeSH), Transgenes (MeSH).
MESH :
- chemical , chemistry : Alkenes.
- chemical , genetics : Acetyltransferases, Oxidoreductases Acting on CH-CH Group Donors, Plant Proteins.
- chemical , metabolism : Acetyltransferases, Eugenol, Glycosides, Oxidoreductases Acting on CH-CH Group Donors.
- metabolism : Plant Leaves, Plants, Genetically Modified, Populus.
- Chromatography, Gas, Gene Expression Regulation, Plant, Genetic Engineering, Tandem Mass Spectrometry, Transgenes.

Abstract

Eugenol, a volatile phenylpropene found in many plant species, exhibits antibacterial and acaricidal activities. This study attempted to modify the production of eugenol and its glycosides by introducing petunia coniferyl alcohol acetyltransferase (PhCFAT) and eugenol synthase (PhEGS) into hybrid aspen. Gas chromatography analyses revealed that wild-type hybrid aspen produced small amount of eugenol in leaves. The heterologous overexpression of PhCFAT alone resulted in up to 7-fold higher eugenol levels and up to 22-fold eugenol glycoside levels in leaves of transgenic aspen plants. The overexpression of PhEGS alone resulted in a subtle increase in either eugenol or eugenol glycosides, and the overexpression of both PhCFAT and PhEGS resulted in significant increases in the levels of both eugenol and eugenol glycosides which were nonetheless lower than the increases seen with overexpression of PhCFAT alone. On the other hand, overexpression of PhCFAT in transgenic Arabidopsis and tobacco did not cause any synthesis of eugenol. These results indicate that aspen leaves, but not Arabidopsis and tobacco leaves, have a partially active pathway to eugenol that is limited by the level of CFAT activity and thus the flux of this pathway can be increased by the introduction of a single heterologous gene.

DOI: 10.1016/j.bbrc.2013.05.060
PubMed: 23707945

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 002582
to stream Main, to step Curation: 002582

Le document en format XML

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<term>Chromatography, Gas (MeSH)</term>
<term>Eugenol (metabolism)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Genetic Engineering (MeSH)</term>
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<term>Oxidoreductases Acting on CH-CH Group Donors (genetics)</term>
<term>Oxidoreductases Acting on CH-CH Group Donors (metabolism)</term>
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<term>Plants, Genetically Modified (metabolism)</term>
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<term>Acetyltransferases (métabolisme)</term>
<term>Alcènes (composition chimique)</term>
<term>Chromatographie en phase gazeuse (MeSH)</term>
<term>Eugénol (métabolisme)</term>
<term>Feuilles de plante (métabolisme)</term>
<term>Génie génétique (MeSH)</term>
<term>Hétérosides (métabolisme)</term>
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<term>Oxidoreductases acting on CH-CH group donors (métabolisme)</term>
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<term>Protéines végétales (génétique)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Spectrométrie de masse en tandem (MeSH)</term>
<term>Transgènes (MeSH)</term>
<term>Végétaux génétiquement modifiés (métabolisme)</term>
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<term>Glycosides</term>
<term>Oxidoreductases Acting on CH-CH Group Donors</term>
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<term>Régulation de l'expression des gènes végétaux</term>
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<front><div type="abstract" xml:lang="en">Eugenol, a volatile phenylpropene found in many plant species, exhibits antibacterial and acaricidal activities. This study attempted to modify the production of eugenol and its glycosides by introducing petunia coniferyl alcohol acetyltransferase (PhCFAT) and eugenol synthase (PhEGS) into hybrid aspen. Gas chromatography analyses revealed that wild-type hybrid aspen produced small amount of eugenol in leaves. The heterologous overexpression of PhCFAT alone resulted in up to 7-fold higher eugenol levels and up to 22-fold eugenol glycoside levels in leaves of transgenic aspen plants. The overexpression of PhEGS alone resulted in a subtle increase in either eugenol or eugenol glycosides, and the overexpression of both PhCFAT and PhEGS resulted in significant increases in the levels of both eugenol and eugenol glycosides which were nonetheless lower than the increases seen with overexpression of PhCFAT alone. On the other hand, overexpression of PhCFAT in transgenic Arabidopsis and tobacco did not cause any synthesis of eugenol. These results indicate that aspen leaves, but not Arabidopsis and tobacco leaves, have a partially active pathway to eugenol that is limited by the level of CFAT activity and thus the flux of this pathway can be increased by the introduction of a single heterologous gene.</div>
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<Abstract><AbstractText>Eugenol, a volatile phenylpropene found in many plant species, exhibits antibacterial and acaricidal activities. This study attempted to modify the production of eugenol and its glycosides by introducing petunia coniferyl alcohol acetyltransferase (PhCFAT) and eugenol synthase (PhEGS) into hybrid aspen. Gas chromatography analyses revealed that wild-type hybrid aspen produced small amount of eugenol in leaves. The heterologous overexpression of PhCFAT alone resulted in up to 7-fold higher eugenol levels and up to 22-fold eugenol glycoside levels in leaves of transgenic aspen plants. The overexpression of PhEGS alone resulted in a subtle increase in either eugenol or eugenol glycosides, and the overexpression of both PhCFAT and PhEGS resulted in significant increases in the levels of both eugenol and eugenol glycosides which were nonetheless lower than the increases seen with overexpression of PhCFAT alone. On the other hand, overexpression of PhCFAT in transgenic Arabidopsis and tobacco did not cause any synthesis of eugenol. These results indicate that aspen leaves, but not Arabidopsis and tobacco leaves, have a partially active pathway to eugenol that is limited by the level of CFAT activity and thus the flux of this pathway can be increased by the introduction of a single heterologous gene.</AbstractText>
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{{Explor lien
   |wiki=    Bois
   |area=    PoplarV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:23707945
   |texte=   Enhancement of production of eugenol and its glycosides in transgenic aspen plants via genetic engineering.
}}

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Enhancement of production of eugenol and its glycosides in transgenic aspen plants via genetic engineering.

Enhancement of production of eugenol and its glycosides in transgenic aspen plants via genetic engineering.

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